Bacterial Lipopolysaccharide Activates Toll-Like Receptor 2 and Toll-Like Receptor 4 Gene Expression in PBMC of Vechur Cattle

Vechur cattle, an indigenous breed of Kerala and it is the smallest cattle in world. They are highly disease resistant. The occurrence mastitis very Rare this as compared to crossbred cattle. Rearing these more cost effective they require less feed. Therefore, characterisation factors involved immune system breeds might provide insight into mechanisms variation resistance. Toll-like receptors (TLRs) part innate immunity, can recognize particular pathogens through Pathogen Associated Molecular pattern s (PAMPs) play important roles host defense. TLR2 TLR4 TLR mediate responsiveness bacterial lipopolysaccharide (LPS). Since susceptible mastitis, vitro expression assay TLRs were accessed by challenging Peripheral Blood Mononuclear Cells (PBMC) with LPS. Treatment PBMC LPS, significantly increased genes (p≤0.01) when that control Among two studied, relative mRNA was relatively higher for (6.90) than (4.24). 2 4might contribute maximum response against bacteria